Part:BBa_K3523008
J23116-rbs-Leader sequence-SOD-rbs-katA-Membrane Protein Ag43_700
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2294
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2166
Illegal NgoMIV site found at 2710
Illegal NgoMIV site found at 2731
Illegal AgeI site found at 2470
Illegal AgeI site found at 2884
Illegal AgeI site found at 3126 - 1000COMPATIBLE WITH RFC[1000]
BBa_K3523008 is used to express a fusion protein, helping to display it on the cell membrane surface. The fusion protein contains superoxide dismutases (SOD) and catalase,. The SOD is a group of enzymes that catalyze the dismutation of superoxide radicals (O2−) to molecular oxygen (O2) and hydrogen peroxide (H2O2), providing cellular defense against reactive oxygen species. The function of catalase is decomposing hydrogen peroxide into water and oxygen; serves to protect cells from the toxic effects of hydrogen peroxide. The promoter J23116 is used to initiate transcription and translation of the fusion protein.
Contribution
In order to eliminate ROS of the intestinal tract more efficiently and rapidly, we further designed the fusion expression of superoxide dismutase (SOD) and catalase(CAT) with membrane proteins (Ag43)^9 of E. coli, so that the enzymes responsible for superoxide degradation were displayed on the surface of the cell membrane. This strategy can promote the contact of enzymes and substrates and accelerate the process of ROS degradation by engineered bacteria theoretically. We have constructed plasmids for this purpose and will further test this hypothesis.
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